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Objective:To analyze and identify ginseng miRNA. Methods: The miRNA and target genes of Shizhu ginseng and Yuan ginseng were detected by the degradation sequencing technology; Functional annotation of Degradome genes was carried out using public databases of KEGG/NR/GO database; The expression of miRNA and target genes of Shizhu ginseng and Yuan ginseng was determinated by real time fluorescence quantitative PCR technique. Results: A total of 13 target genes of eight miRNA families were obtained; The target gene type of miRNA was mainly transcriptional factor, response factor, and signal transduction pathway by means of KEGG/NR/GO database analysis. The results of real time fluorescence quantitative PCR verification of aqc-miR-159, bdi-miR162, cpa-miR319, pgi-miR4376, smo-miR396 and its target genes were basically consistent with the expression of miRNA and target genes from the degradation group. Conclusion: The target genes of partial Panax ginseng miRNA is clarified, which lays the foundation for further study of the possible function of ginseng miRNA.
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Objective: To investigate the relationship of the clustered regularly interspaced short palindromic repeats (CRISPR) with resistance genes and virulence genes. Methods: The Shigella genome sequences, resistance genes and virulence genes sequences were obtained from GenBank Database. Then we obtained the distribution of CRISPR1, CRISPR2 and CRISPR-Q1 and the information of repeat and spacers in Shigella by multiple sequences alignment. Meanwhile, the distribution of resistance genes and virulence genes in Shigella were obtained by BLAST and the relationship of the CRISPR with resistance genes and virulence genes was computed by SPSS 17.0. Results: The spacers of CRISPR1 and CRISPR2 showed obvious differences in different Shigella strains, and the CRISPR-Q1 was more widely distributed (over 99%). The virulence genes were widely distributed in Shigella (more than 56%); the distribution of resistance genes (blaOXA-1, camr, Sul2, and tetB) was also wide (over 50%). There were significant differences between the CRISPR1/CRISPR2 and 9 kinds of virulence genes and 4 kinds of resistance genes (P<0.05). There were significant differences between the CRISPR-Q1 and 3 kinds of virulence genes and 4 kinds of resistance genes (P<0.05). There were significant differences between the CRISPR1/CRISPR2, CRISPR-Q1 and the number of virulence genes and resistance genes (P<0.05). There were significant differences between the CRISPR1/CRISPR2 and the number of spacers of CRISPR-Q1 (χ2=61.6, P<0.001). Conclusion: The distribution of CRISPR was quite different in Shigella, and the number of spacers was also different. There were negative correlation of CRISPR of Shigella with some resistance genes and virulence genes. The CRISPR1/CRISPR2 had a positive relationship with the number of the spacers.
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Objective: To verify the existence of microRNAs (miRNAs) extracted from fresh ginseng decoction. Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. MiR-159 and miR-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction. Results: Ginseng miRNA were successfully extracted from fresh decoction. MiR-159 and miR-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng. Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.
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<p><b>OBJECTIVE</b>To explore the levels of T-lymphocyte subsets and IL-17, IL-35, IFN-γ in peripheral blood of patients with multiple myeloma(MM) and their clinical significance.</p><p><b>METHODS</b>A total of 86 MM patients in our hospital from January 2014 to January 2017 were enrolled in MM group and 30 healthy persons were enrolled in control group, the CD4/CD8T cells ratio, CD4CD25CD127Treg level in peripheral blood were detected by flow cytometer. The levels of IL-17, IL-35 and IFN-γ in peripheral serum were detected by ELISA, and the differences of detected indicators between different groups were compared.</p><p><b>RESULTS</b>Compared with control group, the proportion of CD4T cells and CD4/CD8T cells ratio decreased, the proportion of CD8T cells and Treg increased in MM group. The differences of T lymphocyte subsets level between group III stage of MM and control group were statistically significant (P<0.05). With enhancing of clinical stages, Treg level showed a increasing trend, especially in III stage (P<0.05), the serum level of IL-17 as followed in turn: III stage>II stage>I stage>control, the serum level of IL-35 and IFN-γ as followed in turn: control>I stage>II stage>III stage (P<0.05). In terms of disease status, the propurtion of Treg cells as fllowed in turn: disease progression stage>stable stage>control (P<0.05), the serum level of IL-17 as followed in turn also: disease progression stage>stable stage (P<0.05), while the serum level of IL-35 and IFN-γ as followed in turn: control>disease table stage>progression stage (P<0.05).</p><p><b>CONCLUSION</b>The abnomal level of T-lymphocyte subsets, Treg, IL-17, IL-35 and IFN-γ are related with progression and prognosis of MM patients.</p>
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<p><b>OBJECTIVE</b>To analyze the metabolic levels of energy and substance in chronic superficial gastritis (CSG) patients of Pi deficiency syndrome (PDS) and of Pi-Wei hygropyrexia syndrome (PWHS), including lipid, protein, nucleic acid, carbohydrate, trace element, and energy metabolism, and to study the pathogenesis mechanism of PDS from substance and energy metabolisms.</p><p><b>METHODS</b>Recruited were 8 CSG patients who visited at First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine and Guangdong Provincial Hospital of Traditional Chinese Medicine from June 2004 to March 2005, including 4 patients of PDS and 4 of PWHS. Their gastric mucosae were used for experiments of DNA microarray. The dual-channel DNA microarray data were bioinformatically analyzed by BRB ArrayTools and IPA Software.</p><p><b>RESULTS</b>Obtained were fifty-six differentially expressed genes involved in substance and energy metabolisms with the expression fold more than 2, including 11 genes up-regulated and 45 genes down-regulated. Of them, genes correlated to lipid metabolism included CRLS1, LRP11, FUT9, GPCPD1, PIGL, SULT1A4, B3GNT1, ST8SIA4, and ACADVL, mainly involved in the metabolic processes of fatty acid, cholesterol, phospholipids, and glycolipid. Genes correlated to protein metabolism included ASRGL1, AARSD1, EBNA1BP2, PUM2, MRPL52, C120RF65, PSMB8, PSME2, UBA7, RNF11, FBXO44, ZFYVE26, CHMP2A, SSR4, SNX4, RAB3B, RABL2A, GOLGA2, KDELR1, PHPT1, ACPP, PTPRF, CRKL, HDAC7, ADPRHL2, B3GNT1, ST8SIA4, DDOST, and FUT9, mainly involved in the biosynthesis processes of protein, ubiquitination, targeted transport and post-translation modification. Genes correlated to nucleic acid metabolism included DFFB, FLJ35220, TOP2A, SF3A3, CREB3, CRTC2, NR1D2, MED6, GTF2IRD1, C1ORF83, ZNF773, and ZMYND11, mainly involved in DNA replication and repair, transcription regulation. Genes correlated to carbohydrate metabolism included AGL, B3GNT1, FUT9, ST8SIA4, SULT1A4, DDOST, and PIGL, mainly involved in glucogen degradation and glycoconjugate biosynthesis. Genes correlated to trace element metabolism included COMMD1, SLC39A6, FTL, CHRFAM7A, SCGN, and S100A6, mainly involved in ion metabolisms of copper, zinc, ferri, and calcium. Genes correlated to energy metabolism included AK3 and COX7B, mainly involved in mitochondria structure and oxidative phosphorylation processes.</p><p><b>CONCLUSION</b>The metabolic levels of energy and substance including lipid, protein, nucleic acid, carbohydrate, and trace element were obviously reduced in patients of PDS, which might be an important pathogenesis mechanism for its occurrence.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Energy Metabolism , Genetics , Gastritis , Diagnosis , Genetics , Metabolism , Gene Expression Regulation , Medicine, Chinese Traditional , Oligonucleotide Array Sequence Analysis , TranscriptomeABSTRACT
<p><b>OBJECTIVE</b>To determine the bioinformatical characteristics of differential gene expression in patients with chronic superficial gastritis (CSG) with the Pi-deficiency syndrome (PDS) and those of the non-Pi-deficiency syndrome (non-PDS), i.e. patients of CSG with Pi-Wei dampnese-heat syndrome and healthy persons.</p><p><b>METHODS</b>With the BRB-Array Tools software package, original data collection and bioinformatic: analysis of gene arrays were conducted in 6 CSG patients of PDS (CSG-PDS), 6 CSG patients of non-PDS (CSG-nPDS), and 6 healthy volunteers (Normal).</p><p><b>RESULTS</b>Compared with non-PDS, the gene expressions: in PDS with regards to protein synthesis, energy metabolism, immune reaction and ionic transport tended to be down-regulated, while those concerning secretion, cytoskeleton and ubiquitinization were up-regulated dominantly.</p><p><b>CONCLUSIONS</b>The two kinds of samples, CSG-PDS/Normal and CSG-PDS/CSG-nPDS, have their respective gene expression profiles with different characteristics. Gene expression profile has certain referential significance in syndrome classification.</p>
Subject(s)
Humans , Chronic Disease , Cluster Analysis , Computational Biology , Gastritis , Genetics , Oligonucleotide Array Sequence Analysis , SyndromeABSTRACT
Objective To explore the appropriate ways and contents of reproductive health education for middle school students and to understand reproductive health related sexual behavior and influencing factors among middle school students. Methods Reproductive health related sexual behavior was evaluated among junior and senior middle school students in Luoyang by cluster sampling. The statistical software of SAS 8.1 was adopted for data analyses. Sexual behavior and influencing factors were analyzed by logistic regression. Results Critical sexual behaviors were found significantly higher in senior students,including masturbation, sexual fantasy and sexual intercourse than that in junior students (P<0.05), and boys had higher prevalence than girls (P<0.05). Results from multivariate logistic regression model analyses indicated that incidence rate of sexual behavior among those who ever having had experiences was higher than those who were inexperienced ( OR = 2.62,95 % CI:1.21-5.66). Incidence rate of sexual behavior was related to access of reproductive health and STD/AIDS knowledge ( OR = 3.09,95 % CI:1.43-6.51). In addition, incidence rate of sexual behavior was related to attitude and relation of amour between boys and girls ( OR = 2.24,95 % CI:1.32-3.75). Conclusion Awareness on reproductive health knowledge among middle school students was not enough. Marginal sexual behaviors as masturbation and sexual fantasy had not been correctly and openly discussed to avoid inappropriate sexual activities.